Protein concentration and filtration

Download the application note to see how inline refractive index (RI) measurement supports tighter control of protein concentration and buffer exchange—without stopping the process to take manual samples.

In this application note, you learn how to:

• Monitor protein concentration in real time during ultrafiltration and diafiltration (UF/DF)
• Track buffer exchange progress and identify endpoint conditions with greater confidence
• Reduce reliance on manual sampling to support more consistent operations
• Select recommended installation points for best performance in filtration systems
• Understand practical considerations such as background signal compensation and calibration approaches

UF/DF is critical to final yield and product quality, but concentration and buffer exchange can be difficult to control with periodic offline testing. Inline RI measurement provides continuous visibility so teams can make timely adjustments, improve consistency between batches, and support documented process control.

The application note also includes links to two peer-reviewed papers showing the use of Vaisala refractometers in this application.

Receive the full application note by email by completing the form.

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