In this webinar you'll get the combination of measurement expertise and real-world downstream process experience. Yik Lam brings hands-on knowledge from downstream purification development and manufacturing technology transfer, with a focus on harvest, normal flow filtration, and tangential flow filtration. He is also an author on peer-reviewed work evaluating the use of a Vaisala's Polaris refractometer as a PAT tool to monitor and control UF/DF and SPTFF operations at commercial scale.
Together, Tamara and Yik will discuss how inline RI in modern protein concentration processes can be a game-changer: processes are intensified, titers are higher, SPTFF is more widely used, and PAT expectations continue to grow. UF/DF and TFF remain high-risk steps for yield and quality, despite this, protein concentration is still often estimated, delayed, or operator-dependent.
UF/DF and SPTFF are increasingly expected to deliver tighter control, higher yield, and more robust process performance — but many teams still rely on offline measurements or inferred estimates to make critical concentration decisions. This webinar shows how inline refractive index measurement can make protein concentration visible while the process is still running, so decisions can be made when they still matter.
In this webinar, you will learn how to:
- Understand the measurement gap in modern UF/DF and SPTFF processes, where concentration changes rapidly and traditional methods may be delayed or operator-dependent
- See how inline RI measurement can support:
- more accurate endpoint detection
- improved yield and quality consistency
- retentate homogeneity verification
- earlier detection of process deviations and membrane fouling
- more informed real-time decisions in UF/DF and SPTFF
- Learn how RI differs from traditional concentration measurement methods and why it adds real value without adding unnecessary complexity
- Explore approaches to buffer matrix compensation, including:
- buffer-specific calibration for applications where the buffer remains stable
- dual RI measurement concepts for applications where the buffer changes dynamically, such as diafiltration
- Hear how refractometry has been used in published work as a process analytical technology for real-time monitoring and control of commercial-scale TFF operations using Vaisala inline process refractometers
- Review practical implementation considerations, from R&D to GMP manufacturing, including calibration strategy, installation points, and integration options
